{"id":286,"date":"2019-10-10T21:42:40","date_gmt":"2019-10-10T16:12:40","guid":{"rendered":"https:\/\/medicineplexus.com\/?p=286"},"modified":"2019-10-10T21:42:40","modified_gmt":"2019-10-10T16:12:40","slug":"cpcsea-guidelines","status":"publish","type":"post","link":"https:\/\/medicineplexus.com\/cpcsea-guidelines\/","title":{"rendered":"CPCSEA guidelines"},"content":{"rendered":"\n<p><strong>CPCSEA guidelines<\/strong><\/p>\n\n\n\n<ul><li>Committee for the purpose of control and supervision of experiments on animals. <\/li><li>CORE MEMBER: Hon. Smt. <strong>Maneka Gandhi<\/strong> \u2013 drafted under chairperson<\/li><li>Headquarters at Chennai<\/li><\/ul>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"467\" height=\"351\" src=\"https:\/\/medicineplexus.com\/wp-content\/uploads\/2019\/10\/image-87.png?w=467\" alt=\"\" class=\"wp-image-287\" srcset=\"https:\/\/medicineplexus.com\/wp-content\/uploads\/2019\/10\/image-87.png 467w, https:\/\/medicineplexus.com\/wp-content\/uploads\/2019\/10\/image-87-300x225.png 300w\" sizes=\"(max-width: 467px) 100vw, 467px\" \/><\/figure><\/div>\n\n\n\n<p><strong>IAEC\ncomposition<\/strong> <\/p>\n\n\n\n<ul><li>A Biological Scientist.<\/li><li>Two Scientists from different\nbio disciplines. <\/li><li>Veterinarians involved in the care\nof Animals. <\/li><li>Scientist In-Charge of Animal\nHouse Facility. <\/li><li>The Chairman (preferably Head\nof the Institution \/ Department) and <\/li><li>Member Secretary needs to be\nnominated from the above five members. <\/li><li>Other members: Main Nominee,\nLink Nominee, Scientist from outside and Socially Aware Nominee nominated by\nCPCSEA.<\/li><li>The validity of IAEC is for 3\nyears.<\/li><\/ul>\n\n\n\n<p><strong>FUNCTION OF CPCSEA <\/strong><\/p>\n\n\n\n<ul><li>Registration of establishments\nconducting animal experimentation or breeding of animals for this purpose.<\/li><li>Selection and appointment of\nnominees in the Institutional Animal Ethics Committees of registered\nestablishments. <\/li><li>Approval of Animal House\nFacilities on the basis of reports of inspections conducted by CPCSEA. <\/li><li>Permission for conducting\nexperiments involving the use of animals. <\/li><li>Recommendation for import of\nanimals for use in experiments. <\/li><li>Action against establishments\nin case of violation of any legal norm\/stipulation.<\/li><\/ul>\n\n\n\n<p><strong>Objectives <\/strong><\/p>\n\n\n\n<ul><li>to promote the <strong>humane care of animals used<\/strong> in biomedical and behavioral research and testing. <\/li><li>To <strong>avoid unnecessary pain<\/strong> before, during and after the experiment. <\/li><li>To provide <strong>guideline for -housing, care, breeding, and maintenance -the source of experimental animals<\/strong> -acceptable experimental procedures for <strong>anesthesia and euthanasia<\/strong><\/li><\/ul>\n\n\n\n<p><strong>Veterinary care <\/strong><\/p>\n\n\n\n<ul><li>Adequate veterinary care must\nbe provided and is the responsibility of a <strong>veterinarian<\/strong>\nor a person who has <strong>training or\nexperience &#8216;in laboratory animal sciences and medicine<\/strong>. <\/li><li>Observed regularly for the <strong>sign of illness, injury, or abnormal\nbehavior <\/strong><\/li><li>Contagious disease <strong>\u2013 isolated from a healthy animal.<\/strong><\/li><li>can also contribute to the\nestablishment of appropriate policies and procedures<\/li><\/ul>\n\n\n\n<p><strong>Animal procurement <\/strong><\/p>\n\n\n\n<ul><li>All animals must be <strong>acquired lawfully<\/strong> as per the CPCSEA\nguidelines. <\/li><li>A <strong>health surveillance program for screening incoming animals<\/strong> should\nbe carried out to assess animal quality. <\/li><li><strong>Methods of transportation<\/strong> should also be\ntaken into account. <\/li><li>animals should be <strong>quarantined and stabilized<\/strong> according to\nprocedures appropriate for the species and circumstances.<\/li><\/ul>\n\n\n\n<p><strong>QUARANTINE<\/strong><\/p>\n\n\n\n<ul><li><strong>Separation of newly received animals from those already in the facility<\/strong> until the health and possibly the microbial status of newly received animals has been determined.<\/li><li>The minimum duration of quarantine for a <strong>small animal-1 week<\/strong>, and <\/li><li>for a <strong>larger animal-6 week.<\/strong> <\/li><li>An effective quarantine <strong>minimizes the chance for the introduction of pathogens<\/strong> into an established colony.<\/li><\/ul>\n\n\n\n<p><strong>STABILIZATION AND SEPARATION <\/strong><\/p>\n\n\n\n<ul><li><strong>Physiologic, psychological and nutritional stabilization<\/strong> should be given before their use. <\/li><li><strong>The duration<\/strong> of stabilization will <strong>depend on the type and duration of animal\ntransportation, and species of animal. <\/strong><\/li><li>Physical separation of the animal\nby species is recommended to prevent interspecies disease transmission and to\neliminate anxiety and possible physiological and behavioral changes due to\ninterspecies conflict. <\/li><li>It shall be <strong>acceptable to house different species in\nthe same room, e.g. two species have a similar pathogen status and are behaviorally\ncompatible<\/strong>.<\/li><\/ul>\n\n\n\n<p><strong>SURVEILLANCE, DIAGNOSIS, TREATMENT, AND CONTROL of DISEASE<\/strong> <\/p>\n\n\n\n<ul><li>All animals should be observed\nfor <strong>signs of illness, injury, or\nabnormal behavior<\/strong> by animal house staff. <\/li><li>Animals that show signs of a\ncontagious disease should be isolated from healthy animals in the colony.<\/li><\/ul>\n\n\n\n<ul><li><strong>PERSONAL HYGIENE<\/strong> <\/li><li>Animal care staff maintains a\nhigh standard of personal cleanliness. <\/li><li>It is acceptable to use\ndisposable gear such as <strong>gloves, masks,\nhead covers, coats, coveralls, and shoe covers. <\/strong><\/li><li>Personnel should not be\npermitted to eat, drink, smoke or apply cosmetics in animal rooms.<\/li><\/ul>\n\n\n\n<p><strong>TRANSPORT OF LABORATORY ANIMALS<\/strong> <\/p>\n\n\n\n<ul><li>main considerations -&gt; \u2022 the mode of transport, \u2022 the containers, the animal density in cages, food, and water during transit, \u2022 protection from transit infection, injuries and stress.<\/li><\/ul>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"654\" height=\"268\" src=\"https:\/\/medicineplexus.com\/wp-content\/uploads\/2019\/10\/image-88.png?w=654\" alt=\"\" class=\"wp-image-288\" srcset=\"https:\/\/medicineplexus.com\/wp-content\/uploads\/2019\/10\/image-88.png 654w, https:\/\/medicineplexus.com\/wp-content\/uploads\/2019\/10\/image-88-300x123.png 300w\" sizes=\"(max-width: 654px) 100vw, 654px\" \/><\/figure><\/div>\n\n\n\n<p><strong>DURATIONS\nOF EXPERIMENTS<\/strong><\/p>\n\n\n\n<p><strong>No\nanimal<\/strong> should be used for experimentation for <strong>more than 3 years<\/strong> unless adequate\njustification is provided.<\/p>\n\n\n\n<p>Multiple surgical procedures on a single\nanimal for any testing or experiment are not to be practiced unless specified\nin a protocol only approved by the IAEC.<\/p>\n\n\n\n<p><strong>LABORATORY ANIMAL: HUSBANDRY &amp; MANAGEMENT<\/strong><\/p>\n\n\n\n<p><strong>PHYSICAL FACILITIES <\/strong><\/p>\n\n\n\n<p>Professional judgment must\nbe exercised when developing a practical system for animal care.<\/p>\n\n\n\n<p>\u00d8 Specialized\nlaboratories<\/p>\n\n\n\n<p>\u00d8 Individual areas\ncontiguous with or near animal housing areas for such activities as surgery,<\/p>\n\n\n\n<p>intensive care, necropsy, radiography, preparation of special diets, experimental manipulation, treatment, and diagnostic laboratory procedures containment facilities or<\/p>\n\n\n\n<p>\u00d8 Equipment, if hazardous biological, physical, or chemical agents are to be used<br>\u00d8 Receiving and storage areas for food, bedding<br>\u00d8 Pharmaceuticals and biologics and supplies<br>\u00d8 Space for administration, supervision, and direction of the facility<br>\u00d8 Showers, sinks, lockers and toilets for personnel<br>\u00d8 An area for washing and sterilization of equipment and supplies,<br>\u00d8 An autoclave for equipment<br>\u00d8 Food and bedding and separate areas<br>\u00d8 For holding soiled and unclean equipment<br>\u00d8 An area for repairing cages and equipment<br>\u00d8 An area to store wastes prior to incineration or removal<\/p>\n\n\n\n<ul><li><strong>BUILDING MATERIAL <\/strong><strong>\u00e0 <\/strong>moisture-proof, fire-resistant,\nseamless materials are most desirable for interior surfaces including vermin\nand pest resistance. <\/li><\/ul>\n\n\n\n<ul><li><strong>CORRIDOR-<\/strong> wide enough to facilitate the\nmovement of personnel as well as equipment and should be kept clean. <\/li><\/ul>\n\n\n\n<ul><li><strong>UTILITIES<\/strong>&#8211; water lines, drain pipes and\nelectrical connection <\/li><\/ul>\n\n\n\n<ul><li><strong>ANIMAL ROOM DOORS<\/strong>&#8211; rust, vermin, and\ndustproof. it properly within their frames and provided with an observation\nwindow. <\/li><\/ul>\n\n\n\n<ul><li><strong>Windows<\/strong> are not recommended for small\nanimal facilities. However, where power failures are frequent and backup power\nis not available, they may be necessary to provide an alternate source of light\nand ventilation<\/li><\/ul>\n\n\n\n<ul><li><strong>FLOORS<\/strong>&#8211; smooth, moisture-proof,\nnon-absorbent, skid-proof.<\/li><\/ul>\n\n\n\n<ul><li><strong>WALLS &amp; CEILINGS-<\/strong> free of cracks,\nunsealed utility penetrations, or imperfect junction with doors, ceilings,\nfloors, and corners. <\/li><\/ul>\n\n\n\n<ul><li><strong>STORAGE AREAS<\/strong>&#8211; separate storage areas\nshould be designed for feed, bedding, cages, and materials not in use. <\/li><\/ul>\n\n\n\n<ul><li><strong>FACILITIES FOR SANITIZING EQUIPMENT AND SUPPLIES-<\/strong> an area for sanitizing cages and ancillary equipment is essential\nwith adequate water supply. <\/li><\/ul>\n\n\n\n<ul><li><strong>EXPERIMENTAL AREA<\/strong>&#8211; should be carried out\nin a separate area from the place where animals are housed.<\/li><\/ul>\n\n\n\n<ul><li><strong>ENVIRONMENT <\/strong>\u2022 Air conditioning is for\nlaboratory animals. <\/li><\/ul>\n\n\n\n<ul><li>\u00a0a temperature within the range of <strong>18 -29 degrees<\/strong> Celcius relative <strong>humidity- 30-70%<\/strong> throughout the year \u2022 for large animal comfortable zone-18-37\u00b0 c, Ventilation designed with <strong>12-15 air cycles per hour.<\/strong><\/li><\/ul>\n\n\n\n<ul><li><strong>POWER &amp; LIGHTING-<\/strong> the electrical\nsystem should be safe and provide appropriate lighting and a sufficient no. of\npower outlets. A time control light system should be used. <\/li><\/ul>\n\n\n\n<ul><li><strong>NOISE CONTROL-<\/strong> noise-free environment<\/li><\/ul>\n\n\n\n<p><strong>ANIMAL HUSBANDRY <\/strong><\/p>\n\n\n\n<ul><li><strong>CAGING &amp; HOUSING SYSTEM<\/strong>&#8211; \uf0fc Adequate\nventilation \uf0fc Meet the biological need of animal \uf0fc Keep the animal dry and\nclean \uf0fc Cages made of steel or painted steel \uf0fc Feeding and watering devices\nshould be easily accessible for filing, changing, cleaning and servicing.<\/li><\/ul>\n\n\n\n<p><strong>the number of the animal which can be housed in a particular cage (of different sizes) can be calculated on the basis of (a) floor area of the cage, (b) recommended floor area per animal and (c) weight<\/strong> <strong>of animal.<\/strong><\/p>\n\n\n\n<ul><li><strong>FOOD <\/strong>\u2022 Should be fed palatable,\nnon-contaminated and nutritionally adequate food. \u2022 Diet should be free from\nheavy metals. <\/li><\/ul>\n\n\n\n<ul><li><strong>BEDDING \uf0a7<\/strong> Absorbent, free of toxic\nchemicals or other substances that could injure animals or personnel \uf0a7 Should\nbe removed and replaced with fresh materials as often as necessary to keep\nanimals clean and dry.<\/li><\/ul>\n\n\n\n<ul><li><strong>WATER <\/strong>\u2022 Ordinarily animals should have\ncontinuous access to fresh, potable, uncontaminated \u2022 drinking water, according\nto their particular requirements. <\/li><\/ul>\n\n\n\n<ul><li><strong>Waste disposal<\/strong> \u2022 Wastes should be\nremoved regularly and frequently. All waste should be collected and \u2022 disposed\nof in a -safe and sanitary manner. The most preferred method of waste disposal\nis incineration. <\/li><\/ul>\n\n\n\n<ul><li><strong>EMERGENCY, WEEKEND AND HOLIDAY CARE<\/strong> \u2022\nAnimal should be cared for by qualified personnel every day, including weekends\nand holidays, to safeguards their well- being including emergency veterinary\ncare<\/li><\/ul>\n\n\n\n<ul><li><strong>Recordkeeping<\/strong> The Animal House should\nmaintain the following records: <\/li><\/ul>\n\n\n\n<ul><li>Animal House plans, which\ninclude a typical floor plan, all fixtures, etc. <ul><li>Animal House staff record &#8211;\nboth technical and non &#8211; technical <\/li><\/ul><ul><li>Health record of staff and animals.\n<\/li><\/ul><ul><li>All SOPs relevant to the\nanimals <\/li><\/ul><ul><li>Breeding, stock, purchase, and\nsales records <\/li><\/ul><ul><li>Minutes of institute Animals\nEthics Committee Meetings<\/li><\/ul><ul><li>Records of experiments\nconducted with the number of animals used (copy of Form b) <\/li><\/ul><ul><li>Death Record <\/li><\/ul><ul><li>The clinical record of sick\nanimals. <\/li><\/ul><ul><li>Training record of staff\ninvolved<\/li><\/ul><\/li><\/ul>\n\n\n\n<p><strong>ANAESTHESIA <\/strong><\/p>\n\n\n\n<ul><li>Unless contrary to the achievement of the results of the study, sedatives, analgesics, and anesthetics should be used to control pain or distress under the experiment<\/li><li>ensured that the anesthesia is given for the full duration of the experiment. <\/li><li>Before using actual anesthetics the animal is prepared for anesthesia by overnight fasting and using pre-anesthetics, which block parasympathetic stimulation of the cardio-pulmonary system and reduce salivary secretion. Atropine is the most commonly used anticholinergic agent<\/li><\/ul>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"654\" height=\"268\" src=\"https:\/\/medicineplexus.com\/wp-content\/uploads\/2019\/10\/image-90.png?w=654\" alt=\"\" class=\"wp-image-290\" srcset=\"https:\/\/medicineplexus.com\/wp-content\/uploads\/2019\/10\/image-90.png 654w, https:\/\/medicineplexus.com\/wp-content\/uploads\/2019\/10\/image-90-300x123.png 300w\" sizes=\"(max-width: 654px) 100vw, 654px\" \/><\/figure>\n\n\n\n<p><strong>Euthanasia (eu =good, thanatos =death) <\/strong><\/p>\n\n\n\n<ul><li>Euthanasia is resorted to\nevents where an animal is required to be sacrificed on termination of an experiment\nor otherwise for ethical reasons. <\/li><li>The procedure should be carried\nout quickly and painlessly in an atmosphere free from fear or anxiety. <\/li><li>For accepting a euthanasia\nmethod as humane it should have an initial depressive action on the central\nnervous system for immediate insensitivity to pain. <\/li><li>The method should in all cases\nmeet the following requirements:<\/li><li>(a) Death, without causing\nanxiety, pain or distress with minimum time lag phase.<\/li><li>(b) Minimum physiological and\npsychological disturbances.<\/li><li>(c) Compatibility with the\npurpose of the study and minimum emotional effect on the operator.<\/li><li>(d) The location should be\nseparate from animal rooms and free from environmental contaminants.<\/li><\/ul>\n\n\n\n<ul><li>Methods \u2022 Physical methods \u2022 electrocution, exsanguination, decapitation, cervical dislocation \u2022 Drug administration overdose of chloral hydrate, ketamine, chloroform<\/li><\/ul>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"654\" height=\"350\" src=\"https:\/\/medicineplexus.com\/wp-content\/uploads\/2019\/10\/image-91.png?w=654\" alt=\"\" class=\"wp-image-291\" srcset=\"https:\/\/medicineplexus.com\/wp-content\/uploads\/2019\/10\/image-91.png 654w, https:\/\/medicineplexus.com\/wp-content\/uploads\/2019\/10\/image-91-300x161.png 300w\" sizes=\"(max-width: 654px) 100vw, 654px\" \/><\/figure><\/div>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"654\" height=\"185\" src=\"https:\/\/medicineplexus.com\/wp-content\/uploads\/2019\/10\/image-92.png?w=654\" alt=\"\" class=\"wp-image-292\" srcset=\"https:\/\/medicineplexus.com\/wp-content\/uploads\/2019\/10\/image-92.png 654w, https:\/\/medicineplexus.com\/wp-content\/uploads\/2019\/10\/image-92-300x85.png 300w\" sizes=\"(max-width: 654px) 100vw, 654px\" \/><\/figure><\/div>\n\n\n\n<p><strong>Institutional\nBiosafety Committee (IBSC)<\/strong> <\/p>\n\n\n\n<ul><li>constituted in all centers\nengaged in genetic engineering research and production activities. <\/li><\/ul>\n\n\n\n<p>The committee will constitute the following.<\/p>\n\n\n\n<ul><li>Head of the institution or his\nnominee<\/li><li>3 or more scientists engaged in\nDNA work or molecular biology with an outside expert in the relevant\ndiscipline.<\/li><li>A member with the medical\nqualification-Biosafety officer<\/li><li>One member nominated by DBT<\/li><li>Any research project which is\nlikely to have biohazard potential during the execution stage or which<\/li><\/ul>\n\n\n\n<p>involves the production of either micro-organisms or\nbiologically active molecules that might cause biohazard should be notified to\nISBC. <\/p>\n\n\n\n<p><strong>The biosafety\nfunctions and activity include the following:<\/strong><\/p>\n\n\n\n<p>I. Registration of Biosafety Committee membership\ncomposition with RCGM and submission of the report.<\/p>\n\n\n\n<p>ISBC will provide half-yearly reports on the ongoing\nprojects to RCGM regarding the observance of the safety guidelines on\naccidents, risks and on deviations if any. <\/p>\n\n\n\n<p>II. Review and clearance of project proposals falling\nunder the restricted category that meets the requirements under the guidelines.<\/p>\n\n\n\n<p>III. Tailoring biosafety program to the level of risk\nassessment<\/p>\n\n\n\n<p>IV. Training of personnel on biosafety<\/p>\n\n\n\n<p>V. Instituting health monitoring program for laboratory\npersonnel<\/p>\n\n\n\n<p>Complete medical check-up of personnel working in\nprojects involving work with potentially dangerous microorganisms should be\ndone prior to starting such projects. <\/p>\n\n\n\n<p>Follow up medical check-ups including pathological test\nshould be done periodically, annually<\/p>\n\n\n\n<p>for scientific workers involved in such projects. Their\nmedical records should be accessible to the RCGM. It will provide half-yearly\nreports on the ongoing projects to RCGM regarding the observance of the safety\nguidelines on accidents, risks and on deviations if any.<\/p>\n\n\n\n<p>VI. Adopting emergency plans.<\/p>\n\n\n\n<p><strong>So far the biosafety\ncommittee has been already set up in 124 institutions.<\/strong> The other institutions will be asked to take similar action.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>CPCSEA guidelines Committee for the purpose of control and supervision of experiments on animals. CORE MEMBER: Hon. Smt. Maneka Gandhi \u2013 drafted under chairperson Headquarters at Chennai IAEC composition A Biological Scientist. Two Scientists from different bio disciplines. Veterinarians involved in the care of Animals. Scientist In-Charge of Animal House Facility. The Chairman (preferably Head[&#8230;]\n","protected":false},"author":3,"featured_media":287,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","footnotes":""},"categories":[3],"tags":[],"_links":{"self":[{"href":"https:\/\/medicineplexus.com\/wp-json\/wp\/v2\/posts\/286"}],"collection":[{"href":"https:\/\/medicineplexus.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/medicineplexus.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/medicineplexus.com\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/medicineplexus.com\/wp-json\/wp\/v2\/comments?post=286"}],"version-history":[{"count":0,"href":"https:\/\/medicineplexus.com\/wp-json\/wp\/v2\/posts\/286\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/medicineplexus.com\/wp-json\/wp\/v2\/media\/287"}],"wp:attachment":[{"href":"https:\/\/medicineplexus.com\/wp-json\/wp\/v2\/media?parent=286"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/medicineplexus.com\/wp-json\/wp\/v2\/categories?post=286"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/medicineplexus.com\/wp-json\/wp\/v2\/tags?post=286"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}